期刊论文

Determination of Cadmium in the Water by Fluorometry with Tetraiodofluorescein Sodium Salt and 1, 10-phenantroline

中文

中南医学科学杂志

2095-1116

2014

42

6

579-581+629

10.15972/j.cnki.43-1509/r.2014.06.010

本校为第一机构

目的 建立一种荧光光谱测定水中镉的新方法. 方法 在pH =6.0的乙酸-乙酸钠(HAe-NaAc)缓冲介质中,镉(Cd2+)与四碘荧光素钠、邻啡哆啉反应形成络合物,体系的最大激发波长为525 nm,发射波长为531 nm,在一定范围内,其荧光强度与Cd2+的浓度成线性关系. 结果 Cd2+浓度在3.92×10 6mol/L ～2.00 ×10-5 mol/L范围内,荧光强度变化值(△F)与镉离子浓度(cCd2+)有良好的线性关系,线性方程为△F=58.9c(×10-5 mol/L) +4.8,相关系数r=0.999 2,检出限为1.91×10-6 mol/L,样品加标回收率为86.6％～101.5％.结论 本方法可直接用于水中镉的测定,选择性好,准确可靠,结果满意.

Objective To establish a new method of fluorescence spectroscopy to determine trace cadmium in water. Methods At pH = 6.0 HAc-NaAc buffer medium, the complex with Cd2＋ , Tetraiodofluorescein sodium salt and 1,10- Phenanthroline hydrate was formed. The results showed that with maximum wavelength for 525 nm and the emission wavelength for 531 nm there was a good linear relationship between the fluorescence intensity and the concentration of Cd2＋. Results Among the range of 3.92 × 10^- 6 mol/L -2.00 ×10^-5 mol/L,the linear equation was AF = 58...