期刊论文

中文

中南医学科学杂志

2095-1116

2014

4

348-350

10.15972/j.cnki.43-1509/r.2014.04.002

湖南省自然科学基金资助项目(编号14JJ2091)； 湖南省教育厅资助科研项目(12C0339)；

本校为第一机构

目的：观察miR-19b对巨噬细胞 ABCA1表达的影响。方法在 THP-1源性巨噬细胞中转染miR-19b模拟物(mimic )和抑制物(inhibitor),荧光定量PCR检测ABCA1 mRNA,Western blot检测ABCA1蛋白水平。结果转染miR-19b mimic后,巨噬细胞ABCA1 mRNA及蛋白水平均显著下调；转染miR-19b inhibitor后ABCA1蛋白则明显上调,但ABCA1 mRNA无变化。结论 miR-19b下调巨噬细胞ABCA1的表达。

Objective To investigate the impact of miR-19b on the expression of macrophage ABCA1. Methods The protein and mRNA levels of ABCA1 were detected by Western blot and quantitative real time PCR ( qRT-PCR) respec-tively in THP-1 macrophage transfected with miR-19b mimic and inhibitor. Results The protein and mRNA levels of ABCA1 were obviously downregulated in THP-1 macrophage transfected with miR-19b mimic. The protein level of ABCA1 was evidently upregulated in THP-1 macrophage transfected with miR-19b inhibitor,while ABCA1 mRNA level had no sig-nificantly alterna...