期刊论文

The Effect of Sjcb2 in Resistance to Re-infection of Schistosoma Japonicum

中文

中南医学科学杂志

2095-1116

2014

42

1

11-16

10.15972/j.cnki.43-1509/r.2014.01.011

30972576:国家自然科学基金 11JJ9022:湖南省自然科学基金-衡阳市联合基金

本校为第一机构

目的 构建日本血吸虫组织蛋白酶B(Sjcb2)真核表达载体,检测日本血吸虫组织蛋白酶B2单独和联合吡喹酮(PZQ)在小鼠抗血吸虫再感染中的作用.方法 将60只BALB/c雌性小鼠随机均分为六组,建立日本血吸虫感染动物模型:感染组、感染攻击组、空质粒处理组、Sjcb2免疫组、吡喹酮处理组、Sjcb2联合吡喹酮处理组.Sjcb2免疫组和Sjcb2联合吡喹酮组分别在初次尾蚴感染前第21天、14天及7天接种pcDNA3.1(+)/Sjcb2重组质粒,空质粒处理组接种pcDNA3.1(+)空质粒.初次尾蚴感染后第六周,对吡喹酮处理组和Sjcb2联合吡喹酮处理组小鼠给予PZQ治疗.除感染组外其它各组在第八周再次进行尾蚴攻击感染.分别于第8周和第14周取各...

Objective To construct the eukaryotic expression plasmid pcDNA3.1（＋）/Sjcb2 and evaluate the effect of Schistosoma japonicum cathepsin B2 alone or combined with praziquantel （PZQ）in resisting the reinfection of Schistosoma japonicum. Methods The infected mouse model of Schistosoma japonicum was constructed and employed.Female BALB/ c mice were divided into six groups：the infected group of Schistosoma japonicum,the re-infected challenged group with cercaria after first infected,the pcDNA3.1（＋） treated group,the pcDNA3.1（＋）/Sjcb2 treat...