期刊论文

Effect of Orchidopexy on Bcl-2 Expression and Spermatogenic Cell Apoptosis in Rat Experimental Cryptorchidism

中文

中南医学科学杂志

2095-1116

2013

41

3

229-232

10.15972/j.cnki.43-1509/r.2013.03.024

2011SK3088:湖南省科技厅项目 2011KJ6,2010KJ03:衡阳市科技局项目

本校为第一机构

目的 研究实验性大鼠隐睾复位固定术对Bcl-2表达及生精细胞凋亡的影响.方法 24只雄性SD大鼠建立左侧隐睾模型,右侧睾丸切断引带后还纳入阴囊作为假手术组;模型建立7天后实施左侧隐睾复位固定术,隐睾复位固定术前、术后第24天及48天分别处死大鼠8只,采用免疫组织化学检测各组睾丸Bcl-2表达的变化,运用TUNEL法检测各组睾丸生精细胞凋亡.结果隐睾内生精细胞凋亡增加,复位固定术能降低生精细胞凋亡,增强睾丸内Bcl-2表达.假手术组凋亡指数(4.86%±0.27%)与复位固定术后48天组(5.59%±0.67%)比较差异无显著性(P＞0.05),但均低于复位固定术后24天组(14.48%±0.70%)及复位固定术前的隐睾组(29.45%±1.19...

Objective To investigate effect of orchidopexy on spermatogenic cell apoptosis and Bcl-2 expression in rat experimental cryptorchidism.Methods Unilateral left cryptorchidism was surgically induced and the right testis（as Sham group） was stayed into the scrotum after gubernaculum cut in each of 24 male SD rats（age of 21 days）.On the 7th day after the establishment of cryptorchidism model,8 rats were sacrificed（as Cryp group）,and the others were operated with orchidopexy of left testis.On the 24th and 48th day after orchidopexy,8 rats were ...