期刊论文

中文

中南医学科学杂志

2095-1116

2013

41

3

225-228

10.15972/j.cnki.43-1509/r.2013.03.008

国家自然科学基金资助项目（81200218,81170278,81070220）； 衡阳市科技局课题（2011KJ11）；

本校为第一机构

目的 研究核因子-κB(NF-κB)抑制剂二硫代氨基甲酸吡咯烷(PDTC)对THP-1巨噬细胞脂质蓄积和胆固醇流出的影响.方法 60 nmol/L PMA孵育THP-1巨噬细胞24 h后,分为3组:对照组、氧化型低密度脂蛋白(ox-LDL)组(100 μg/mL)和PDTC组(100 μg/mL ox-LDL+50 μmol/L PDTC).用油红O染色观察细胞内脂质蓄积情况,高效液相色谱分析法检测细胞内胆固醇水平,用液体闪烁计数法观察细胞内胆固醇的流出.结果 PDTC抑制ox-LDL诱导的THP-1巨噬细胞脂质蓄积,明显降低细胞内总胆固醇、游离胆固醇和胆固醇酯的含量,增加载脂蛋白A-Ⅰ(apoA-Ⅰ)介导的胆固醇流出,但对HDL介导的胆固醇流出无明显影响.结论 PDTC抑制ox-LDL诱...

Objective To investigate the effect of NF-κB inhibitor pyrrolidinedithiocarbamate（PDTC） on lipid accumulation and cholesterol efflux in THP-1 macrophages induced by ox-LDL.Methods Human THP-1 macrophage was induced using phorbol-12-myristate acetate（PMA,160 nmol/L） for 24 h.Then cells were divided into three groups：control group,ox-LDL group（100 μg/mL） and PDTC group（100 μg/mL ox-LDL＋50 μmol/L PDTC）.Oil red O staining was used to observe the intracellular lipid droplets.Cellular cholesterol was determined by high performance liqui...