期刊论文

Effects of Arecoline on the Proliferation and Differentiation of 3T3-L1 Preadipocytes

中文

中南医学科学杂志

2095-1116

2013

41

1

7-9,27

10.15972/j.cnki.43-1509/r.2013.01.002

基金项目：本课题受囝家自然科学基金（81000328）和南华大学博士科研启动基金（2010XQD39）资助.

本校为第一机构

目的 探讨槟榔碱对3T3-L1前脂肪细胞增殖、分化的影响及其机制.方法 采用四甲基偶氮唑盐(MTT)法和油红 O染色检测不同浓度(10、30、50、100 μmol /L)的槟榔碱对3T3-L1前脂肪细胞增殖和分化的影响,荧光定量PCR检测过氧化物酶体增殖物激活受体γ(PPAR γ)和CAA T/增强子结合蛋白α(C/ EBP α)的表达.结果 10~100 μmol /L 槟榔碱作用 3T3-L1前脂肪细胞12 h和24 h能促进3T3-L1前脂肪细胞增殖,且呈一定的量效关系.到分化的第9天,与对照组相比,50 μmol /L 槟榔碱组胞浆中的脂滴明显减少,同时PPAR γ和C/ EBP α mRNA表达水平显著降低.结论 槟榔碱能促进3T3-L1前脂肪细胞的增殖、抑制其分化,其机制可...

objectives To investigate the effects of arecoline on the proliferation and differentiation of 3T3-L1 pread- ipocytes and to elucidate its possible mechanism. Methods 3T3-L1 preadipocytes were cultured and treated with differ- ent concentrations of arecoline （ 10,30,50,100 μmol/L） , the proliferation and differentiation of 3T3-L1 preadipoeytes were evaluated by MTTmethods and oil red O staining ,respectively. The expressions of peroxisome proliferation activated receptorα （PPAR α） and CAAT/ enhancer binding protein （C/ EBP α） mRNAs we...