期刊论文

中文

中南医学科学杂志

2095-1116

2013

41

2

127-130

10.15972/j.cnki.43-1509/r.2013.02.010

衡阳市科学技术发展计划项目（2010KJ15）；

本校为第一机构

目的 观察KLF4过表达对小鼠巨噬细胞(RAW264.7)胆固醇蓄积的影响及对ATP结合盒转运蛋白A1 (ABCA1)表达的影响. 方法 构建KLF4过表达的RAW264.7细胞株.将细胞分为三组:对照组、ox-LDL组、KLF4过表达+ox-LDL组,后两组加入终浓度为30 μg/mL的ox-LDL处理24 h.分别运用油红O染色观察细胞质脂滴变化,高效液相色谱法(HPLC)检测细胞内总胆固醇(TC)和游离胆固醇(FC)含量,Westem blot法检测各组细胞ABCA1及KLF4蛋白表达. 结果 泡沫细胞模型组的细胞内存在大量的红色脂滴,KLF4及ABCA1蛋白表达及胆固醇含量较对照组升高；KLF4过表达细胞经ox-LDL处理后,细胞内脂滴较模型组明显减少,胆固醇含量下降,ABCA1蛋白...

Objective To investigate cholesterol accumulation and expression of ATP binding cassette transporter A1 （ ABCA1 ） in RAW264. 7 ＇affected by Kruppel-like factor 4 （KLF4）. Methods Stable subline of RAW264.7 cells over-expressed KLF4 were established. RAW264.7cells were induced into foam cells by 30 p,g/ml oxidized low density lipoprotein （ox-LDL）for 24 h. Lipid droplets in cytoplasm were observed by Red oil O staining. The cellular contents of total cholesterol （TC） and free cholesterol （ FC ） were examined by high performance liquid c...