期刊论文

中文

中南医学科学杂志

2095-1116

2012

40

2

123-126

10.15972/j.cnki.43-1509/r.2012.02.004

81100212/H0215:国家自然科学基金 11C1094:湖南省教育厅科学研究项目

本校为第一机构

目的 探讨过表达CAMP反应元件结合蛋白(CREB)在内皮细胞氧化损伤中的作用. 方法 建立H2O2诱导的血管内皮细胞损伤模型,将内皮细胞ECV304分为对照组、H2O2组、转染PCI空载体组、转染PCI空载体+ H2O2组、转染PCI-CRESAP组和转染PCI-CREB+H2O2组,采用MTT法检测细胞存活率,硫代巴比妥酸检测丙二醛(MDA)含量,黄嘌呤氧化酶法检测细胞超氧化物歧化酶(SOD)活性. 结果 转染CREB+ H2O2组的细胞存活率和SOD活性明显高于H2O2组(P＜0.05),而转染CREB+ H2O2组的MDA含量明显低于H2O2组(P＜0.05). 结论 过表达CREB对H2O2诱导的内皮细胞氧化损伤具有保护作用.

Objective To investigate the effect of overexpression of CREB on oxidative injury in endothelial cells.Methods The endothelial cell oxidative injury model was induced by H2O2.ECV304 cells were divided into 6 groups：control group,H2O2 group,PCI transfection group,PCI transfection＋H2O2 group,PCI-CREB transfection group and PCI-CREB transfection＋H2O2 group.The viablity of ECV304 cells was detected by MTT.The MDA concentration was observed by TBA method.Levels of SOD were tested by xanthine oxidase.Results Compared with H2O2 group,both cell viab...