期刊论文

中文

南华大学学报(自然科学版)

1673-0062

2011

25

1

87-91

10.19431/j.cnki.1673-0062.2011.01.019

湖南省教育厅基金资助项目（07C634）；

本校为第一机构

在pH 7～8的弱碱性条件下,丹酰氯可与芹菜素反应生成一种酚酯化合物,使丹酰氯的内源性荧光发生猝灭,其猝灭程度(△F=F0-F)与加入芹菜素的量在一定范围内呈良好的线性关系.在优化的条件下,体系△F与芹菜素的浓度在6.40×10-7mol/L～5.92×10-5mol/L范围内线性关系良好,线性回归方程为△F=112.18CAp(×10-5mol/L)-1.25,相关系数r=0.998 9.检出限为1.92×10-7mol/L,RSD为2.6%～4.1%,加标回收率为98.1%～101.5%.本方法简便、快速,用于芹菜中芹菜素的含量测定结果满意.

After the phenol ester compound generated by apigenin reacting with dansyl chloride in weakly alkaline medium,the endogenous fluorescence of dansyl chloride was quenched,and there was a good linear relationship between the quenching value（ΔF=F0-F） and the concentration of apigenin.And under the optimal conditions,the linear range of calibration curve for determination of apigenin was 6.40×10-7~5.92×10-5 mol/L,and the linear regression equation was ΔF= 112.18CAp（×10-5 mol/L）-1.25 with correlation coefficient 0.9989 and the detection lim...