期刊论文

中文

中南医学科学杂志

2095-1116

2010

38

2

173-176

10.3969/j.issn.2095-1116.2010.02.007

湖南省长沙市科学技术局基金资助（K051155-72）.

本校为第一机构

目的 初步探讨乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒(HBIG-PBCA-NP)的肝靶向性.方法 HBIG-PBCA-NP与HepG2.2.15 细胞及QSG7701细胞共孵育,透射电镜观察细胞内的纳米粒;以HBIG-PBCA-NP、PBCA-NP或HBIG尾静脉注射昆明小鼠,透射电镜观察肝细胞内的纳米粒,免疫组织化学检测肝组织HBIG的含量.结果 透射电镜照片显示HepG2.2.15细胞和QSG7701细胞的胞核和胞浆内可见纳米粒,昆明种小鼠肝组织中可见大量的纳米粒;肝组织HBIG免疫组织化学结果表明相同浓度的HBIG-PBCA-NP组比HBIG组显色更明显(P

Objective To evaluate the liver - targeting of HBIG - PBCA - NP in vitro and in vivo. Methods QSG7701 cells and HepG2.2.15 cells were cultured with DMEM and HBIG -PBCA -NP, the ceils was collected for detection of the nanoparticle by transmission electron microscope （ TEM ） ; HBIG - PBCA - NP, PBCA - NP and HBIG were administrated to the mice by the tail intravenous injection, then the mice were killed and the liver tissiue were collected for detecting the nanopartiele by TEM and HBIG by immunohistochemistry. Results After inoculation of HBIG...