期刊论文

中文

中南医学科学杂志

2095-1116

2010

38

2

177-179

10.3969/j.issn.2095-1116.2010.02.008

本校为第一机构

目的 探讨人参皂甙对副溶血性弧菌引起的肠炎的治疗作用.方法 分别用副溶血性弧菌、副溶血性弧菌加人参皂甙Rb1刺激人结肠癌细胞(Caco2) 4 h后,提取细胞总蛋白,Western blotting 检测其MAPKs信号通路活化情况,另用PBS作用于Caco2细胞作为阴性对照.结果 经副溶血性弧菌刺激后,Caco2细胞内ERK1/2磷酸化作用明显加强,并随副溶血性弧菌剂量增加而增强,p38在该菌作用下也有明显的磷酸化;而人参皂甙Rb1能抑制该菌诱导的ERK1/2和p38信号通路的活化作用.结论 人参皂甙Rb1抑制副溶血性弧菌诱导的Caco2细胞MAPKs中ERK1/2和p38信号通路的活化作用,从而可能缓解其下游细胞因子引起的炎症作用.

Objective To investigate the effects of ginsenoside Rbl on the intestinal inflammation. Methods Caco2 cells were activated with V. parahaemolyticus, V. parahaemolyticus and Rb1 for 4 hours. After activation, total protein was purified and MAPKs signal pathways were checked by Western blotting. Results ERK1/2 pathway was activated by V. parahaemolytieus and increased with the dose of bacteria solution. P38 pathway was also activated by the bacteria. However, Rb1 inhibited V. parahaemolytieus - induced activation of ERK1/2 and p38. Conclusion Rbl might have potential therapeutic effects on eontr...