期刊论文

The Roles of CD28 - and CD40L -mediated Costimulation Played in Chlamydial Urogenital Infection in Mice

中文

中南医学科学杂志

2095-1116

2010

38

4

468-471

10.3969/j.issn.2095-1116.2010.04.006

基金项目：湖南省科技厅基金项目（2009FJ3207） 湖南省教育厅项目（05C460）

本校为第一机构

目的 初步探讨由CD28和CD40L介导的协同刺激信号在衣原体感染中的作用. 方法 用1×104IFUs的沙眼衣原体鼠生物型(MoPn)经生殖道感染雌性C57BL/6J野生型(wt)、CD28 KO、CD80/CD86 KO、CD40L KO及CD40 KO小鼠,免疫荧光法测定生殖道分泌物中衣原体包涵体的数量.初次感染后80天和115天,处死小鼠,分离生殖道,做病理检查;用衣原体EB刺激脾细胞,测定特异性细胞免疫应答水平. 结果 CD40LKO及CD40 KO小鼠阴道带菌时间比wt、CD28 KO及CD80/CD86 KO组明显延长;而CD28 KO及CD80/CD86 KO小鼠在初次感染衣原体后,生殖道病理变化比其他组轻.脾细胞因子水平显示,CD40L KO及CD40 KO组IFN-γ产生量较其它组低. 结论...

Objective To investigate the roles of CD28 - and CD40L - mediated costimulation in the development of protective immunity and pathology during Chlamydial urogenital infection in mice. Methods wt and mice deficient in CD28, CD80/CD86, CIMOL and CD40 were inoculated intravaginally with 1 × 10 4 IFUs of live C. muridarum organisms. The number of Chlamydial inclusion bodies in vaginal swabs was measured by immunofluorescence assay. On D80 or D115 after primary infection, mice were sacrificed, the vaginal tract was isolated for pathology, and the s...