期刊论文

Influences of the SR-BI Expression by HDL,LDL,and ox-LDL in HepG2 Cells

中文

中南医学科学杂志

2095-1116

2010

38

1

10-13+20

10.3969/j.issn.2095-1116.2010.01.003

湖南省教育厅高校重点科研项目（09A078）；

本校为第一机构

目的观察高密度脂蛋白（HDL）、低密度脂蛋白（LDL）及氧化型低密度脂蛋白（ox-LDL）对HepG2细胞B类I型清道夫受体（SR-BI）表达及胆固醇含量的影响。方法用HDL、LDL及ox-LDL处理HepG2细胞,采用RT-PCR、Western印迹检测细胞SR-BI的表达;采用油红O染色及高效液相色谱法（HPLC）观察细胞内脂质的含量。结果HDL、LDL及ox-LDL处理HepG2细胞后,与对照组比较,各脂蛋白处理组细胞SR-BI mRNA及蛋白表达上调,油红O染色发现HDL、LDL及ox-LDL处理组细胞内脂滴含量明显增加;HPLC检测提示,与对照组细胞内总胆固醇含量（43.18±7.80 mg/g蛋白）比较,ox-LDL处理组（113.01±15.90 mg/g蛋白）、LDL处理组（171.1...

Objective To investigate the influences of the SR-BI expression and cholesterol content by ox-LDL,LDL and HDL in HepG2 cell lines. Methods HepG2 cells were co-incubated with 50 μg/mL ox-LDL,LDL and HDL,respectively.SR-BI mRNA and protein level were determined by reverse transcription-polymerase chain reaction and western blot.Cellular cholesterol content was determined by Oil Red O staining and high performance liquid chromatography analysis. Results Ox-LDL,LDL and HDL up-regulated the expression of SR-BI in HepG2 cells compared with control g...