期刊论文

中文

中南医学科学杂志

2095-1116

2009

37

4

397-399

10.3969/j.issn.2095-1116.2009.04.010

本校为第一机构

目的 探讨锌(Zn2+)对体外培养人肝癌细胞HepG2生长的影响及可能机制. 方法 运用3-(4,5二甲基2-噻唑)-2,5-二苯基四氮唑溴盐(MTT)比色法检测不同浓度锌对人肝癌细胞HepG2细胞增值的影响;流式细胞术分析细胞周期和凋亡率;免疫蛋白印迹(Western blot)分析Bax和Bcl-2蛋白表达的差异. 结果 高浓度锌(250 μmol/L)对体外培养的HepG2细胞生长活性有抑制作用,并随Zn2+浓度的递增其细胞存活率下降越明显(P

Objective To explore the effect of Zinc on the viability of HepG2 cells cultured in virto and its mechanism to provide the experiment ground for treating liver cancer. Method The cell vigor was determined by MTT assay, and the Western blot was performed to evaluate the effects of high concentrations on expression of Bax, Bcl - 2. The cell cycle and apoptotic changes were studied by the flow cytometry. Results The results showed that Zinc of high concentration restrained the viability of HepG2 cells. Zinc（250μmol/L） could inhibit the prolifer...