期刊论文

Expression of Inducible Nitric Oxide Synthase and Proinflammatory Cytokines Regulated by Activating Nuclear Factor KB in Human Monocytic Cells Stimulated With Lipid -associated Membrane Proteins of Ureaplasma Urealyticum

中文

中南医学科学杂志

2095-1116

2009

37

2

140-143

10.3969/j.issn.2095-1116.2009.02.006

本校为第一机构

目的分析解脲脲原体（Uu）的脂质相关膜蛋白（LAMPs）对人单核细胞（THP-1）表达诱导型一氧化氮合酶（iNOS）和前炎症因子白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）和白细胞介素6（IL-6）的影响。方法用Uu的LAMPs刺激人单核细胞后,Western blot法检测核因子κB（NF-κB）的激活和iNOS基因的表达,格氏试剂测定NO,ELISA法测定IL-1β、TNF-α和IL-6的含量。结果Uu LAMPs通过激活NF-κB诱导人单核细胞表达iNOS,且能以时间和剂量依赖方式刺激人单核细胞产生NO,诱导表达前炎症因子（IL-1β、TNF-α和IL-6）;NF-κB抑制剂PDTC可抑制NF-κB的激活、iNOS的表达及NO的产生。结论Uu LAMPs通过激活...

Objective To investigate the expression of the inducible nitric oxide synthase （iNOS） gene and proinflammatory cytokines （IL- 1β,TNF-α and IL -6 ） in human monocytic cells （THP - 1 ） stimulated by lipid - associated membrane proteins （LAMPs） prepared from ureaplasma urealyticum （ Uu）. Methods THP -1 was stimulated with Ureaplasma urealytieum LAMPs, the activation of NF - κB or the expression of iNOS was detected by Western blot, the content of nitric oxide （ NO ） was detected by using Griss reagents, and the level of IL - 1β ,TN...