期刊论文

Study on Frequency of HPRT Locus Mutation in Human Bronchial Epithelial Cells Induced by COFC

中文

中南医学科学杂志

2095-1116

2007

35

2

165-167

10.3969/j.issn.2095-1116.2007.02.006

本校为第一机构

目的 研究烹调油烟冷凝物致人支气管上皮细胞HPRT 基因位点突变的影响及二甲基亚砜的拮抗作用,以探讨烹调油烟的致突变性及其防护.方法 采用体外多核细胞法研究烹调油烟冷凝物对人支气管上皮细胞HPRT 基因位点的影响及二甲基亚砜的防护作用.结果 烹调油烟冷凝物诱发人支气管上皮细胞HPRT 基因位点突变, 且突变频率随着染毒浓度的增加而升高, 突变频率与烹调油烟冷凝物浓度之间存在一定的剂量- 效应关系,二甲基亚砜对HPRT 基因位点突变有显著的拮抗作用.结论 烹调油烟冷凝物能诱发人支气管上皮细胞HPRT 基因位点突变,二甲基亚砜能抑制烹调油烟冷凝物的诱变作用.HPRT 基因突变频率可望成为接触环境...

Objective To study the mutational effect of COFC on HPRT gene of human bronchial epithelial cells and protection of DMSO. Methods Cytokinesis- block （CB） assay was used to detect the frequency of HPRT locus mutation induced by COFC and its dose - effect relationship and protection of DMSO. Results The test showed that COFC could induce HPRT locus mutation in human bronchial epithelial and there was a linear relationship between the concentration of COFC and the mutation frequency （MF} of HPRT gene. The mutation could be inhibited effectively...