期刊论文

Acute Toxic Effect of Methyl Tertiary Butyl Ether in Cerebellum of Rat

中文

中南医学科学杂志

2095-1116

2007

35

2

154-157

10.3969/j.issn.2095-1116.2007.02.003

湖南省卫生厅科研项目基金（NoB2005090）；

本校为第一机构

目的 观察甲基叔丁基醚（MTBE）对大鼠小脑皮质神经递质GABA、Na^＋，K^＋-ATP酶、Ca^2＋-ATP酶的影响，探讨甲基叔丁基醚对中枢神经系统的急性毒作用机制。方法 54只健康成年SD大鼠，以染毒前为对照，染毒剂量为1050mg／kg体重，染毒后用免疫组织化学方法检测大鼠小脑皮质神经递质GABA的表达并用相对灰度值定量；用分光光度法测定小脑皮质Na^＋，K^＋-ATP酶、Ca^2＋-ATP酶的活性。结果 与染毒前比较，小脑皮质GABA在MTBE染毒后0．5h时表达开始增加，1．0h达到高峰，2．0h时开始减弱，8．0h时GABA表达恢复到染毒前水平；MTBE染毒后1．0h小脑皮质Na^＋，K^＋-ATP酶、Ca^2＋-ATP酶的活性降低（P〈...

Objective To explore the acute toxic mechanism of methyl ternary butyl ether （MTBE）, by detecting the change of the neumtransmitter γ- amino - butyric acid （ GABA ） and the activity of Na^＋, K^＋ - ATPase, Ca^2＋- ATPase in the cerebellar cortex. Methods 54 adult SD rats were randomly divided into control and experiment groups. The MTBE exposed dose was 1 050 mg/kg, bw and respectively injected by abdominal cavity. The method （SABC method} was used to detect the change of the neurotransmitter GABA in the cerebellar cortex. The spectropho...