期刊论文

Establishment of Human Laryngeal Cancer Cell Line Transfected with Human IL- 24 cDNA and Its Expression

中文

中南医学科学杂志

2095-1116

2007

35

3

389-391,400

10.3969/j.issn.2095-1116.2007.03.025

本校为第一机构

目的 建立转染人白细胞介素-24(hIL-24)基因的喉癌细胞株,并研究hIL-24的表达情况.方法 将携带人白细胞介素-24的质粒peDNA3.1(+)-hIL-24转导入人喉表皮样癌细胞Hep-2中,G418筛选阳性克隆,RT-PCR、Western blot和ELISA法对hIL-24的表达进行检测.结果 IL-24基因成功地转导入Hep-2细胞中并能高效表达.RT-PCR电泳结果显示hIL-24基因在mRNA水平有表达;ELISA法测得106个转染阳性细胞24h内培养上清液中hIL-24的含量为162.8±15.4 pg/mL,空载体转染及未转染的细胞未检测到hIL-24.结论 hIL-24基因成功转染人喉癌细胞系Hep-2细胞且能持续大量表达.

Objective To establish human laryngeal cancer cell line transfected with human IL- 24 cDNA and demonstrate its expression in laryngeal cancer cell. Methods The recombinant expression plasmid pcDNA3. 1（ ＋ ） -hIL- 24 was transfected into human laryngeal cancer cell line Hep- 2 cells and the positive clone was screened by G418.Then the expression of hIL-24 in Hep- 2 cells was tested by RT-PCR and Western blot assay; the secretion of hIL - 24 in medium was detected by ELISA. Results Human IL- 24 cDNA was successfully integrated into Hep - 2 cell...