期刊论文

Inhibition of VEGF Antisense Oligonucleotides on the Growth of Osteosarcoma in Nude Mice

中文

中南医学科学杂志

2095-1116

2006

34

1

21-23

10.3969/j.issn.2095-1116.2006.01.007

湖南省卫生厅课题资助项目（编号4-04-WS-B2004-089）.

本校为第一机构

目的探讨血管内皮生长因子(VEGF)反义寡核苷酸对裸鼠骨肉瘤生长的抑制作用.方法制备BALC/C裸鼠皮下骨肉瘤模型18只,随机分为3组:VEGF反义寡核苷酸(ASODN)治疗组、VEGF正义寡核苷酸(SODN)治疗组及对照组,每组6只.接种MG-63人成骨肉瘤细胞后24 h内,分别皮下注射ASODN及SODN进行治疗,对照组只注射生理盐水,每周2次,连续4周;观察各组裸鼠肿瘤的生长情况、裸鼠的一般情况与生存期.断颈处死裸鼠,游标卡尺测量肿瘤体积大小,天平称量肿瘤的重量.结果与对照组瘤重(7.45±0.60 g)比较,VEGF ASODN组(4.13±0.80 g)能明显抑制裸鼠肿瘤生长(P《0.01),VEGF SODN组(6.92±0.69 g)则无明显抑制作用(P》0.05) . V...

Objective To study the inhibitory effect of VEGF antisense oligonucleotides on the growth of osteo- sarcoma in nude mice. Methods The osteosarcoma cells were cultured and implanted subcutaneously into 18 nude mice which were subsequently divided into three groups： VEGF ASODN group, VEGF SODN group and the control group. Different treatment were given respectively at 24 hours after cell inoculation. The weight and volume of subcutaneous tumors were measured. Results In comparison with the tumor weight of the control group（7.45 ± 0.60 g）, the...