期刊论文

中文

中南医学科学杂志

2095-1116

2005

33

3

357-360

10.3969/j.issn.2095-1116.2005.03.018

本校为第一机构

目的探讨凋亡抑制因子Survivin 在鼻咽癌中的表达及其临床意义.方法用逆转录聚合酶链反应(RT-PCR)和Western blot免疫印迹法,检测2株鼻咽癌细胞株、25例鼻咽癌组织及对应的对侧鼻咽部黏膜、10例正常人的鼻咽部黏膜组织中Survivin mRNA和蛋白的表达.结果 Survivin在正常鼻咽部黏膜组织中不表达.2株鼻咽癌细胞株、80.0%的鼻咽癌组织和40.0%的对应对侧相对正常鼻咽部黏膜中均可检测到Survivin 基因 mRNA和蛋白的表达,Survivin mRNA在鼻咽癌组织中的表达较对应的对侧鼻咽部黏膜组织高,其吸光度(A)比值分别为0.83±0.22和0.29±0.14,差异有显著性(P

Objective To study the expression and clinical significance of apoptosis inhibitor Survivin in nasopharyngeal carcinoma(NPC). Methods The expression level of Survivin mRNA and protein in two human nasopharyngeal arcinoma cell lines, biopsies of 25 NPC and opposite nasopharyngeal mucosa and 10 normal nasopharygeal epithelium were examined by RT-PCR and Western blot. Results No Survivin was detected in normal nasopharygeal epithelium. However, the expression of Survivin mRNA and protein was found in two NPC cell lines,80.0% NPC and 40.0% opposite...