期刊论文

Comparative Study on Antimicrobial Activity of Chitosan and Chitooligosaccharide

中文

中南医学科学杂志

2095-1116

2005

33

3

331-333

10.3969/j.issn.2095-1116.2005.03.010

本校为第一机构

目的研究用改良双氧水降解法制备的壳寡糖和前体物质壳聚糖的抗微生物活性并进行比较,提供应用依据.方法采用琼脂稀释法测定壳寡糖和壳聚糖对四种供试菌的抑杀作用;用ELISA检测对HBsAg的破坏效果.结果壳寡糖和壳聚糖均显示明显的抗菌活性,随浓度的增高抗菌效果增强.当壳寡糖浓度为2%时对金黄色葡萄球菌和大肠埃希菌的抑菌率是100%,对枯草杆菌芽孢和白假丝酵母菌的抗菌作用较弱.壳聚糖的抗菌活性稍优于壳寡糖,在测试浓度范围内可完全抑制四种供试菌的生长.但两者对HBsAg均无破坏效果.结论壳寡糖和壳聚糖均具有良好的抗菌活性,但壳聚糖的抗菌活性稍优于壳寡糖.

Objective To compare antimicrobial activity of chitosan with chitooligosaccharide produced by improved H2O2 degradation in vitro. Methods The effect of chitooligosaccharide and chitosan on killing and inhibiting four tested microorganisms was measured by the agar-dilution technique. ELISA was carried out to test their destructive effects on HBsAg. Results Chitosan and chitooligosacchride markedly inhibited growth of most tested microorganisms. Their antimicrobial activities were strengthened with concentration increasing.The inhibitory rate...