期刊论文

A Rapid Method for Extracting Human Genomic DNA From Whole Blood

中文

中南医学科学杂志

2095-1116

2005

32

2

230-232

10.3969/j.issn.2095-1116.2004.02.032

本校为第一机构

目的建立一种简便、快速、有效、微量外周血基因组DNA的提取方法.方法采用NaI作细胞裂解剂裂解细胞和细胞核,用氯仿∶异戊醇(24∶1)直接提取基因组DNA.结果该方法提取外周血基因组DNA的纯度高,A260nm/A280nm=1.83±0.13,每毫升外周血可得DNA 33±3.52 μg.结论本法简便、快速、有效,适用于批量临床标本的处理.

Objective To establish a rapid simple,efficient method for extracting human genomic DNA from whole blood. Method Whole blood is lysed by adding a chaotropic agent NaI,then genomic DNA is directly extracteded with chloroform:isoamyl alcohol(24∶1). Result The genomic DNA extracted with this method is of large molecular weight and A 260 nm/280 nm =1.83±0.13,33.5±3.55 μg DNA can be gained from per milliliter whole blood.The results of PCR and restriction endonucleases digestion for the DNA are good. Conclusion The method is simple, rapid ,effi...