期刊论文

中文

中南医学科学杂志

2095-1116

2001

29

04

348-350+352

10.3969/j.issn.2095-1116.2001.04.004

本校为第一机构

目的探讨解脲支原体（Uu）在液体培养基中的最佳营养条件和在固体培养基上形成菌落的最佳气体条件。方法采用不同的pH值、不同的酵母及小牛血清含量配制解脲支原体培养基，将法国Merieum生产的培养基作对比研究。结果解脲支原体生长最适pH值为6.0 ～6 .5；10％酵母提取液、10％小牛血清能加速解脲支原体的生长，90％N2、5%～10％CO2 能促进解脲支原体菌落生长。结论本研究为改进解脲支原体的培养质量、开发高效的支原体培养基及药敏检测板提供实验依据。

Objective:To investigate the optimum nutrient conditions of liquid media for growth and the optimum air conditions for colony formation on solid culture media of Ureaplasma Urealyticum(Uu). Methods:The media was made adopting different pH value?yeast extract and fetal-bovine serum,with Merieum media made in France as control. Results:The optimum pH value for growth of Uu is 6.0～6.5 ;10% yeast extract and 10%fetal-bovine serum can enhance the growth of Uu. Conclusions:This study provides experimental evidences for both the quality improvement ...