期刊论文

中文

中南医学科学杂志

2095-1116

2001

29

5

435-438

10.3969/j.issn.2095-1116.2001.05.001

南华大学资助 ;编号为 5 -0 0 -XJ-0 0 -0 6 5；

本校为第一机构

目的将人Daxx(human Daxx,hDaxx)及其6种删除突变体(△hDaxx)在原核细胞大肠杆菌BL21(E.coli)中表达,以便在体外探索hDaxx的生物学活性.方法用PCR方法从Hela细胞cDNAs文库中扩增全序列hDaxx cDNA,利用载体pQE3x构建了hDaxx及其△hDaxx质粒,通过异丙基-β-D-硫代半乳糖苷(IPTG)诱导E.coli表达相应蛋白质,并将其纯化.结果 IPTG能诱导hDaxx及其△hDaxx在E.coli中表达,表达产物纯化率达85%以上.结论 hDaxx及其6种△hDaxx能在E.coli中表达.

Objective:To question the biological activities of human Daxx(hDaxx)in vitro,hDaxx and its 6 deletion mutants(△hDaxx)were induced expression in prokaryotic cells,E.coli BL21.Methods:The prokaryotic expression plasmids of hDaxx and △hDaxx were constructed in pQE3x.Expression of hDaxx and △hDaxx were induced by IPTG.Expression productions were purifed using Ni-NTA agarose.Results:Coomassie strains and Western blot showed that hDaxx and △hDaxx were induced expression by IPTG.Purific rates of expression are more than 85%.Conc...