The circumsporozoite protein gene fragment was amplified by polymerase chain reaction and cloned into M13 bacteriophage.Then,the nucleotide sequence of the gene fragment was determined by the dideoxy chain termination method.Using PCGENE software to compare and analyze the CS protein gene sequences of the different isolates of the Plasmodium falciparum.The results demonstrated the polymorphism of CS protein genes in different isolates and different degree variation of the CS protein gene sequences among Plasmodium falciparum PFD-3/YN(fr...
