期刊论文

The Study of Expression of Nerve Growth Factor Receptor TrkA Protein in Spared Dorsal Roots Ganglia of Adult Rats After Partial Dorsal Roots Rhizotomy

中文

中南医学科学杂志

2095-1116

1998

4

371-373

本校为第一机构

为探讨背根节中神经生长因子（ＮＧＦ）及ｔｒｋＡ蛋白对脊髓可塑性的作用，采用成年大鼠５只，建立单侧备用根模型（即切断一例Ｌ１￣４，Ｌ６￣Ｓ１的脊髓节段背根，保留Ｌ５为备用根及备用背根节）。术后动物存活５天，灌流固定，取双侧Ｌ５背根节（ＤＲＧ）冰冻切片，用兔抗２．５ＳＮＧＦ及ｔｒｋＡ蛋白多克隆抗体分别进行Ｓ－Ｐ法免疫组化反应，ＤＡＢ显色。并对免疫反应阳性神经元进行图像分析。其结果手术侧ＤＲＧ中ＮＧＦ及

For exploration of the effect of NGF and trkA protein in plasticity of spinal cord, five adult male SD rats were used to establish spared dorsal root preparation(i.e rhizotomy of unilateral L 1～4 , L 5～S 1,hind limb dorsal roots, leaving L 5 as a spared dorsal root). The rats were perfused and fixed with 4% paraformaldehyde after five days. The L 5 dorsal root ganglia were sectioned and 20 um thickness frozen sections were cut. The sections were immunostained with 2.5 s NGF and trkA protein polyclonal antibody respectinely, the S-P kit was us...