期刊论文

STUDIES ON APOLIPOPROTEIN C OF VERY LOW DENSITY LIPOPROTEIN AMONG THE COLLEGE STUDENTS

中文

中南医学科学杂志

2095-1116

1991

02

89-97

本校为第一机构

本实验分析了50例(男20;女30)正常青年学生 VLDLapoC 各亚组分的百分含量及其在各亚组分间的比值。采用不连续密度梯度角转头超速离心分离脂蛋白法分离 VLDL。于脱脂后对 apoVLDL 进行圆盘法等电聚焦电泳(IEF)。测得 apoVLDLCⅢ_0、CⅡ、CⅢ_1和 CⅢ_2的等电点分别为4.93,4.78,4.72和4.54。用双波长薄层色谱扫描仪扫描等电聚焦电泳分离的 VLDLapoC 区带。结果 VLDLapoCⅡ,CⅢ_0,CⅢ_1和 CⅢ_2的百分含量分别为:22.42±6.94%,18.32±6.87%,30.41±10.61%和27.85±12.11%。无明显的性别差异(p>0.05)。VLDLapoCⅡ/CⅢ_0,CⅡ/CⅢ_1,CⅡ/CⅢ_2,CⅢ_0/CⅢ_1,CⅢ_0/CⅢ_2和 CⅢ_1/CⅢ_2分别为1.44±0.6...

The isoforms of apolipoprotein C(ape C)in very low density li- poprotein Among 50 healthy college students(male:20,female:30) were studied.Very low density lipoproteins were prepared by discon- tinuous density gradient ultracenfugation in angular rotor.The iso- forms of apolipoprotein C were separated by poiyacrylamide gel iso- electric focusing.The isoelectric points of apolipoprotein CⅢ_0 and CⅡ,CⅢ_1,CⅢ_2 determined were 4.93,4.78,4.72 and 4.54 respectively. The percentage values of apolipoprotein C isoform were measured with the SHIMADZU...