期刊论文

中文

环境与健康杂志

1001-5914

2009

26

5

445-447

10.16241/j.cnki.1001-5914.2009.05.005

湖南省教育厅科研项目（402JY02C401）；

本校为第一机构

目的 研究吖啶橙-溴酸钾-萘酚体系的光谱性质及其共振光散射增强机制,建立共振光散射法测定人尿中痕量萘酚新方法.方法在稀硫酸介质中,萘酚与溴酸钾、吖啶橙(acridine orange,AO)发生反应形成聚集离子,导致共振光散射(resonance light scattering,RLS)显著增强,产生新的RLS光谱.研究了反应体系的吸收、荧光、RIS光谱特征,适宜的反应条件.结果 α-萘酚和β-萘酚最大的RLS峰均位于468.0 nm处,线性范围分别为1.41×10~(-7)～2.80×10_(-5) mol/L,1.28×10~(-7)～3.00×10~(-5) mol/L,相关系数分别为0.999 6,0.999 3,检出限分别为0.422×10~(-7) mol/L和0.385×10~(-7) mol/L.RSD为4.8%～7.3%,平均回...

Objective To understand the spectroscopic property of AO-H2SO4-KBrO3-naphthol system and the enhanced mechanism of resonance light scattering （RLS）, and to develop a method for the determination of trace naphthols in urine. Methods In the dilute H2SO4 medium, naphthols could react with KBrO3 and AO to form ion-association complexes, which produced a new RLS spectrum and resulted in the great enhancement of RLS. The characteristics of RLS spectrum, three-dimensions fluorescence spectrum, absorption spectrum and fluorescence spectrum and the op...