期刊论文

中文

环境与健康杂志

1001-5914

2007

24

3

162-164

10.16241/j.cnki.1001-5914.2007.03.016

本校为第一机构

目的 研究二甲基亚砜(DMSO)对烹调油烟冷凝物(COFC)致人支气管上皮细胞遗传毒性作用的影响.方法 采用永生化人支气管上皮细胞系BEAS-2B细胞,分为空白对照组、DMSO对照组、试剂对照组(0.1%乙醇)、COFC染毒组(50、100、150 mg/L)和相应的DMSO保护组(0.5%DMSO).采用单细胞凝胶电泳和微核、多核实验研究COFC的遗传毒性及DMSO对烹调油烟损伤的防护作用.结果 COFC染毒组可引起细胞DNA断裂,细胞拖尾率、彗星尾部DNA含量、尾长、尾部面积和尾距增加,微核多核率升高,DMSO对以上损伤有显著的拮抗作用.结论 COFC对人支气管上皮细胞具有明显的遗传毒性作用,而DMSO对此有抑制作用.

Objective To study the antagonism of DMSO against the toxicity of cooking oil fume condensation to BEAS-2B cell.Methods The comet assay,micronucleus test and multinucleated cells test were used to research the genotoxicity induced by cooking oil fume condensation(COFC)and the antagonism of DMSO.Results COFC induced DNA broken,the tail area,rate of comet occurrence,tail length,tail moment,olive tailmoment increased significantly,the frequencies of micr...