期刊论文

中文

中国急救医学

1002-1949

2014

34

4

358-360

10.3969/j.issn.1002-1949.2014.04.018

湖南省自然科学基金资助项目（08JJ5003）

本校为第一机构

目的研究硫化氢对肺纤维化大鼠的治疗作用及对肺组织中程序性死亡因子-1 (PD-1)表达的影响。方法36只SD大鼠,随机分为对照组、模型组和硫氢化钠干预组。模型组和干预组气管内注射博来霉素诱导肺纤维化,对照组在相同条件下给予生理盐水。第2天起干预组大鼠每天经尾静脉注射硫氢化钠(3. 12 mg/kg),其余两组相同条件下给予生理盐水。治疗第7天和第28天,处死动物取出肺组织,进行病理学观察;用免疫组化和RT-PCR观察各组肺组织PD-1蛋白及mRNA表达的水平。结果病理学观察显示,与模型组比较,干预组肺泡炎和纤维化程度均减轻;与模型组比较,干预组PD-1蛋白及mRNA表达显著降低。结论硫化氢可能通过抑制PD -...

Objective To investigate the effect of hydrogen sulfide on PD -1 expression in the lung of rats with experimental pulmonary fibrosis .Methods Thirty six male SD rats were randomly divided into three groups：the control group , the pulmonary fibrosis group and the treatment group .The model of pulmonary fibrosis was produced by bleomycin endotracheally , while the control group was given saline under the same condition .From the second day the rats in the treatment group were treated with 3.12 mg/kg NaHS （ total volumn of 2 ml） ig, while the o...