期刊论文

Effect of endogenous hydrogen sulfide on autophagy induced by beryllium sulfate in human bronchial epithelial cells

中文

毒理学杂志

1002-3127

2019

33

3

189-193

10.16421/j.cnki.1002-3127.2019.03.003

81573193:国家自然科学基金 CX2018B629:湖南省研究生科研创新项目 2018KYZ042:南华大学研究生科学基金项目 2018KYY221:南华大学研究生科学基金项目

本校为第一机构

目的探讨内源性硫化氢(H_2S)对硫酸铍(BeSO_4 )诱导人支气管上皮细胞(16HBE)自噬的影响.方法用0、100、150、200、250和300 μmol /L BeSO_4处理16HBE细胞48 h,MTT法测定细胞活力,亚甲基蓝分光光度法测定细胞内H_2S含量,Western blot测定自噬标志蛋白LC3-B、Beclin-1及P62表达;用300 μmol /L硫氢化钠(NaHS)或10 mmol /L DL-炔丙基甘氨酸(PPG)预处理16HBE细胞6 h后,用BeSO_4处理细胞,研究H_2S含量对上述指标的影响.结果(1) BeSO_4作用16HBE细胞48 h的半数致死浓度为249 μmol /L;与对照组相比,BeSO_4组自噬蛋白LC3-II /LC3-I和Beclin-1表达升高,细胞活力、H_2S含量和P62蛋白表达降低,当BeSO_4浓...

Objective To investigate the effect of endogenous hydrogen sulfide (H_2S) on Beryllium sulfate (BeSO_4 ) -induced autophagy in human bronchial epithelial cells (16HBE). Methods 16HBE cells were dealt with different concentrations of BeSO_4 (0, 100, 150, 200, 250 and 300 μmol /L) for 48 hours. MTT assays were used to detect the viability of 16HBE cells. The contents of hydrogen sulfide were evaluated with methylene blue assay. Western blot was used to determine the expression of autophagy-associated protein LC3-B,Beclin- 1 and P62.16HBE cells w...