期刊论文

中文

毒理学杂志

1002-3127

2009

23

5

364-367

10.16421/j.cnki.1002-3127.2009.05.004

本校为第一机构

目的 研究醋酸铅能否诱导人肾小管上皮细胞(HK-2)凋亡,并探讨其凋亡机制.方法 平板克隆实验法检测细胞活力;Annexin V(膜联蛋白V)-FITC(异硫氰酸荧光素)/PI(碘化丙啶)双染法结合流式细胞仪分析细胞凋亡率;荧光化学分光仪分析caspase-3、-8.-9(半胱天冬酶-3、-8、-9)的活性变化;Western bolt(免疫印迹法)检测bcl-2、bax蛋白的表达.结果 铅可抑制HK-2细胞生长活力,随着铅浓度增高,细胞生长活力降低(P＜0.05,P＜0.01);铅使caspase-3、caspase-9的活性增强(P＜0.05,P＜0.01),但对caspase-8的活性无明显影响;caspase-3抑制剂可明显抑制铅诱导的caspase-3的活化,并抑制铅诱导的HK-2细胞凋亡;Western b...

Objective To study the effect of lead acetate on the apoptosis of HK-2 cells and the apoptosis pathway. Methods The viabilities of cells were mediated by colony formation assay. The cell apoptosis was determined by flow cytometric analysis with Annexin-V(FTTC) and Propidium Iodide(PI) . Activities of caspase-3, -8, -9 were determined by colorimetric assay. Western-bolting was utilized to measure the expressions of bcl-2 and bax. Results Colony formation assay results revealed that lead could inhibit the viabilities of cells in does-dependent manner; The rates of apoptosis of lead-treated HK-2 ...