期刊论文

Establishment and application of ICT and PCR detection for Clonorchis sinensis

中文

中国热带医学

1009-9727

2016

16

12

1155-1158

10.13604/j.cnki.46-1064/r.2016.12.02

国家卫生行业科研专项（No.201502021）；

本校为第一机构

目的建立华支睾吸虫的免疫胶体金（ICT）和实时荧光定量PCR检测体系,比较两者检测华支睾吸虫的效果。方法以华支睾吸虫成虫抗原包被胶体金,检测血液样本中的相应抗体,建立华支睾吸虫ICT检测方法;以华支睾吸虫18S r RNA基因作为靶基因,设计RT-PCR的特异性引物和探针,检测粪便样本中的核酸实时荧光定量PCR方法;以两种检测体系与商品化的酶联免疫（ELISA）检测试剂盒平行测定比较,考核检测体系的应用价值。结果对200份华支睾吸虫重点人群样本进行检测,ELISA法检出的华支睾吸虫Ig G抗体阳性例数为20例,阳性率为10.0%;ICT法为16例,阳性率为8.0%,灵敏度为80.0%,特异度为100.0%,符合率为98.0%,ICT和ELI...

Objective To establish an immune colloidal gold technique（ICT） for detecting the IgG antibody of Clonorchis sinensis（C.s）, and a real- time fluorescent quantitative PCR（RT- PCR） for detecting the DNA of C.s, and compare their detection effects. Methods The colloidal gold particles were peridiumed by adult worm antigen of C.s to establish the ICT to detect Ig G antibody of C.s. According to the specific sequence of 18 S rRNA of C.s, the primers and the fluorogenic probe of RTPCR were designed, and then the RT-PCR was build to detect fecal ...