期刊论文

中文

中国热带医学

1009-9727

2013

13

4

400-403

10.13604/j.cnki.46-1064/r.2013.04.013

衡阳市科技局基金资助项目（No.2012KJ24） 湖南省教育厅基金资助项目（No.10C0456）

本校为第一机构

目的探讨新生期脂多糖暴露对睾丸发育及波形蛋白表达和分布的影响。方法选取新生雄性SD大鼠150只,随机分为对照组和实验组,实验组于生后第4～12d期间,隔日皮下注射脂多糖（75μg/kg）,共5次,对照组在相同时间皮下注射等容生理盐水。常规饲养至生后14d、21d、28d、包皮分离日、60d、77d分批取材,采用组织学和免疫组化技术,观察脂多糖对大鼠睾丸发育、波形蛋白的表达与分布的影响。结果①与同龄对照组相比,实验组大鼠生精小管横径变小,生精上皮变薄,细胞排列紊乱,生精细胞与支持细胞分离,脱落至管腔,性成熟以后,管腔内精子生成减少;②对照组波形蛋白阳性染色从基膜围绕支持细胞核形成棕黄色的环,并...

Objective To investigate the effects of neonatal period lipopolysaccharide（LPS）exposure on testicular development and vimentin expression of rats.Methods A total of 150 neonatal Sprague-Dawley male rats were used in this study.The litters were randomly divided into either LPS-treated or saline control groups.These rats were administered 75ug/kg LPS or an equi-volume of non-pyrogenic saline via subcutaneous injection on alternate day from postnatal days 4 to 12.All the rats were then fed conventionally and sacrificed on PND14,PND21,PND28,Prepu...