期刊论文

Role of tumor necrosis factor-α in Chlamydia Muridarum infection in the urogenital tract of mice

中文

南方医科大学学报

1673-4254

2020

40

3

388-393

10.12122/j.issn.1673-4254.2020.03.17

18C0457:湖南省教育厅一般项目 2335:湖南省大学生创新创业训练计划 2018KJ110:重点实验室项目

本校为第一机构

目的探讨肿瘤坏死因子α(TNF-α)在抗衣原体感染免疫以及介导衣原体生殖道病理损伤过程中的作用。方法以5~6周龄雌性C57BL/6J野生型小鼠及TNF-αR基因敲除小鼠为研究对象,野生型和TNF-αR基因敲除两组各15只小鼠,经生殖道感染1×10~4包涵体形成单位的鼠衣原体,初次感染后第56天,每组8只小鼠再次感染同等剂量的鼠衣原体。每隔3~4 d取小鼠生殖道分泌物用于衣原体包涵体数目的检测。于初次感染后第80天处死小鼠,收集小鼠腹腔巨噬细胞,并分离生殖道和脾脏。观察输卵管和子宫角病理损伤程度,并采用盲法对管腔扩张以及炎性细胞浸润程度进行半定量分析;测定小鼠巨噬细胞培养上清中的IL-6、IL-8、IL-1α...

Objective To explore the role of tumor necrosis factor-α(TNF-α)in immune response to urogenital chlamydial infection and urogenital pathology in mice. Methods Fifteen female wild-type(WT)C57BL/6J mice and 15 TNF-α receptor knockout(TNF-αR KO)mice were inoculated intravaginally with 1×10~4 inclusion forming units(IFUs)of live C. muridarum. At 56 days after the first inoculation,8 mice from each group were subjected to a second inoculation at the same dose. Vaginal swabs were taken every 3 or 4 days to detect the number of inclusion bodies o...