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N-6-methyladenosine regulates glycolysis of cancer cells through PDK4

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成果类型:
期刊论文
作者:
Li, Zihan;Peng, Yanxi;Li, Jiexin;Chen, Zhuojia;Chen, Feng;...
通讯作者:
Wang, Hongsheng
作者机构:
[Li, Jiexin; Peng, Yanxi; Wang, Hongsheng; Chen, Feng; Li, Zihan] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China.
[Tu, Jian; Li, Zihan] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.
[Peng, Yanxi] Xiangnan Univ, Dept Basic Med, Chenzhou 423000, Hunan, Peoples R China.
[Chen, Zhuojia] Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Collaborat Innovat Ctr Canc Med, Guangzhou 510060, Peoples R China.
[Lin, Shuibin] Sun Yat Sen Univ, Affiliated Hosp 1, Ctr Translat Med, Guangzhou, Peoples R China.
通讯机构:
[Wang, Hongsheng] S
Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China.
语种:
英文
期刊:
Nature Communications
ISSN:
2041-1723
年:
2020
卷:
11
期:
1
页码:
2578
基金类别:
The authors thank Dr Feng Liu and Prof Junjiu Huang at School of Life Sciences, Sun Yat-sen University for technical support. This research was supported by the National Natural Science Foundation of China (Grant Nos. 81973343, 81673454, 81672608, and 31801197), the Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery (2019B030301005), the Guangdong Provincial Key Laboratory of Construction Foundation (No. 2017B030314030), the Fundamental Research Funds for the Central Universities (Sun Yat-sen University) (Nos.19ykpy130 and 19ykzd24), the Natural Science Foundation of Guangdong Province of China (No. 2020A1515010291), and the China Postdoctoral Science Foundation (No. 2018M643354).
机构署名:
本校为其他机构
院系归属:
药学与生物科学学院
摘要:
AbstractStudies on biological functions of N6-methyladenosine (m6A) modification in mRNA have sprung up in recent years. We find m6A can positively regulate the glycolysis of cancer cells. Specifically, m6A-sequencing and functional studies confirm that pyruvate dehydrogenase kinase 4 (PDK4) is involved in m6A regulated glycolysis and ATP generation. The m6A modified 5′UTR of PDK4 positively regulates its translation elongation and mRNA stability via binding with YTHDF1/eEF-2 complex and IGF2BP3, respectively. Targeted specific demethylation o...

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