Protein domains, catalytic activity, and subcellular distribution of mouse NTE-related esterase

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成果类型：

期刊论文

作者：

Chang, Ping-an*;Wang, Zhan-xiang;Long, Ding-xin;Qin, Wen-zhen;Wu, Yi-jun

通讯作者：

Chang, Ping-an

作者机构：

[Chang, Ping-an; Qin, Wen-zhen] Chongqing Univ Posts & Telecommun, Key Lab Mol Biol, Coll Bioinformat, Chongqing 400065, Peoples R China.

[Chang, Ping-an; Long, Ding-xin; Wu, Yi-jun] Chinese Acad Sci, Mol Toxicol Lab, Inst Zool, Beijing 100080, Peoples R China.

[Wang, Zhan-xiang] Fujian Med Univ, Dept Neurosurg, Hosp Xiamen 1, Xiamen 361001, Peoples R China.

[Long, Ding-xin] Univ S China, Sch Publ Hlth, Hengyang 421001, Peoples R China.

通讯机构：

[Chang, Ping-an] C

Chongqing Univ Posts & Telecommun, Key Lab Mol Biol, Coll Bioinformat, Chongqing 400065, Peoples R China.

语种：

英文

关键词：

NTE-related esterase;Protein domain;Esterase activity;Membrane topology;Endoplasmic reticulum

期刊：

Molecular and Cellular Biochemistry

ISSN：

0300-8177

年：

2010

卷：

339

期：

1-2

页码：

181-190

DOI：

10.1007/s11010-009-0382-0

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30870298, 30600329]; Natural Science FoundationNational Natural Science Foundation of China (NSFC) [2007BB5443]; Chongqing Municipal Education Committee [KJ070510]

机构署名：

本校为其他机构

院系归属：

公共卫生学院

摘要：

A mammalian family of lipid hydrolases, designated "patatin-like phospholipase domain containing (PNPLA)" recently has attracted attention. NTE-related esterase (NRE) as a member of PNPLA is an insulin-regulated lysophospholipase with homology to neuropathy target esterase (NTE). Mouse NRE (mNRE) has a predicted amino-terminal transmembrane region (TM), a putative regulatory (R) domain, and a hydrophobic catalytic (C) domain. In the current study, we described the expression of green fluorescent protein (GFP)-tagged constructs of mNRE and mutant proteins lacking the specific protein domains. E...

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Protein domains, catalytic activity, and subcellular distribution of mouse NTE-related esterase

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