期刊论文

中文

中国生化药物杂志

1005-1678

2009

30

1

24-26

基金项目：湖南省衡阳市科技局研究项目（2007KJ022）

本校为第一机构

目的 初步探讨甲壳低聚糖-硒(COS-Se)抗氧化的可能分子机制.方法 用100μg/mL的COS-Se处理THP-1源性巨噬细胞6,12,24,48 h,运用半定量RT-PCR方法检测其超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)mRNA表达水平的变化.结果 COS-Se处理THP-1源性巨噬细胞24和48 h后,SOD mRNA的表达与对照组比较分别上调了14.4%,16.9%(P

Purpose To study the antioxidant mechanism of chitooligosaccharides-selenium （COS-Se）. Methods THP-l-derived maerophages were cultured and treated with COS-Se （100 μg/mL） for 6, 12, 24 and 48 h respectively. Then SOD, GSH-Px mRNA expression was determined by semi-quantity RT-PCR. Restilts Compared with control, COS-Se significantly increased expression of SOD mRNA after 24 h and 48 h and GSH-Px mRNA expression after 6, 12, 24 and 48 h. Conclusion COS-Se could up-regulated SOD and GSH-Px mRNA expression in macrophage cells in vitro, which c...