Purpose: The aim was to solve the problem of water-insoluble of chitosan, a macro-molecular of polysaccharides. Methods: A water-soluble oligosaccharides of hydrolyzed chitosan was prepared by using the method of directly oxidative degradation by H2O2. The structure of the oligosaccharides of hydrolytic chitosan was determined by analysis of their IR spectroscopy, and the polymerization degree was measured by high performance capillary electrophoresis (HPCE). The effects of temperature and concentration of H2O2 on degradation-required time, pro...