期刊论文

Liu, Zhigang

英文

MOLECULAR MEDICINE REPORTS

1791-2997

2017

16

5

6946-6952

10.3892/mmr.2017.7413

National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81272960]; Key Research Program from the Science and Technology Department of Hunan Province, China [2013WK2010]; Key Research Program from Ministry of Human Resources and Social Security of the People's Republic of China [2016-176]; Hunan Province Key Laboratory of Tumor Cellular and Molecular Pathology [2016TP1015]; Construction Program of the Key Discipline in Hunan Province, China (Basic Medicine Sciences in University of South China)

本校为第一机构

Asthma is a complicated systemic disease of the airways, which is characterized by variable symptoms, including bronchial hyper-responsive-ness, inflammation and airflow obstruction. The prevalence of asthma has increased 2-3-fold over recent decades in developed countries; however, the molecular mechanism of asthma remains unclear. In the current study, the expression of recombinant protein Dermatophagoides farinaeI (Derf I) was induced by isopropyl beta-D-1-thiogalactoside (IPTG) and purified using Ni-NTA. Derf I, an important antigen of asthma, was used to establish the animal model of asth...