期刊论文

Min Lingfeng

中文

化学学报

0567-7351

2011

69

21

2609-2617

国家自然科学基金（No.30770647） 湖南省自然科学基金（No.07JJ3055） 湖南长沙市环境保护局专项资金（No.2010）资助项目

本校为其他机构

为了更好地理解苯并芘（Benzo（a）pyrene,B（a）P）在气道细胞诱导的毒理作用机制.应用二维凝胶电泳（two-dimensional electrophoresis,2-DE）分离B（a）P处理组（B（a）P-A549）、对照组（DMSO-A549）的总蛋白质,图像分析识别差异表达的蛋白质点,基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定差异蛋白质点,Western blot验证差异蛋白热激蛋白27（heat shock protein 27,HSP 27）以及锰超氧化物歧化酶（Mn superoxide dismutase,Mn SOD）的表达,使用0.1,1,10μmol/L B（a）P处理A549细胞,应用Western blot及RT-PCR检测各组细胞中Mn SOD的表达,检测各组细胞中总抗氧化活性（total anti...

In order to better understand the molecular mechanisms of Benzo(a)pyrene [B(a)P] mediated toxicity on airway epithelial cells, comparative proteomics involving two-dimensional gel electrophoresis (2-DE) and MALDI-TOF Mass Spectrometry (MS) was performed on total proteins extracts from B(a)P-A549 and its control cell line DMSO-A549. Then the expression of heat shock protein 27 (HSP 27) and Mn superoxide dismutase (Mn SOD), two of the identified differential expressed protein, was analyzed using western blot. After 0.1, 1, 10 μmol/L B(a)P treatm...