期刊论文

Jiang, B.

中文

中华医学遗传学杂志

1003-9406

2000

17

1

42-46

10.3760/j.issn:1003-9406.2000.01.012

本校为第一机构

目的:了解广州汉族人在vWA31A等6个短串联重复序列(short tandem repeat,STR)位点法医学的有关数据.方法:运用STR-PCR、4%变性聚丙烯酰胺凝胶电泳,结合荧光DNA自动检测技术,对汉族人群6个STR位点的等位基因频率和基因型频率进行了调查,并与其他种族或人群的等位基因频率进行了比较.结果:6个STR位点是vWA31A、TH01、F13A01、FES、TPOX和CSF1PO,所有位点的结果均符合Hardy-Weinberg平衡,且各位点等位基因间无相关性,除vWA31A外,其它5个位点的杂合度均低于白种人和黑种人.6个位点的累积DP=0.99999,PE=0.9708,pM=1.059×10-5.结论:所得到的等位基因频率等数据可为中国汉族人群法医个人识别、亲子鉴定...

Objective: This paper was designed to evaluate 6 short tandem repeats(STR) loci for their use in forensic personal identification. Methods: The allele frequencies and genotype frequencies at 6 tetranucleotide STR loci were determined in a sample of 132 unrelated individuals from a Chinese population of Han nationality in Guangzhou. The PCR products were analysed on 4% denaturing PAGE and detected using fluorescently labelled primers in an automated 377 sequencer(PE). The resultant allele frequencies were compared with other population or race databases. Results: All the 6 loci (vWA31A, TH01, F...