期刊论文

Determination of gentamicin in milk based on gold nanoparticles by resonance light scattering and colorimetry

中文

分析试验室

1000-0720

2017

36

11

1316-1319

10.13595/j.cnki.issn1000-0720.2017.0283

本校为其他机构

在2-(N-吗啉)乙磺酸(MES)介质中,庆大霉素与纳米金通过Au-N键结合形成复合物,使纳米金聚集,并在543 nm处存在明显共振散射峰,在525 nm和680 nm处存在明显可见吸收峰,据此建立了共振光散射光谱法和比色法检测庆大霉素的方法。在最优实验条件下,庆大霉素浓度在9.31 ~74.4 nmol/L范围内,543 nm处共振散射光强度增加值(ΔI_(RLS))与庆大霉素浓度呈现良好的线性关系,其线性回归方程为ΔI=199.5c-131.5(10~(-8)mol/L),r=0.9994。在4.1×10~(-9)~2.2×10~(-5)mol/L浓度范围内时,A_(680)/A_(525)增加值[Δ(A_(680)/A_(525))]也与庆大霉素浓度呈良好的线性关系,其线性回归方程为Δ(A_(680)/A_(525))=0.21...

Two sensitive and rapid new methods for determination of gentamicin（Gen） in milk havebeen developed based on gold nanoparticles （GNPs）by resonance light scattering （RLS） and colorimetry. In MES media solution, Gen and gold nanopartieles form a complex eompoundthrough the Au-N bond, leading to significant resonance scattering peak at 543nm and obviously visible absorption peaks at 525 nm and 680 nm. In the optimal experimental conditions,the AJRLs signalat 543 nmlinearly correlated with the concentration of Gen over the range of 9. 31 - 74...