期刊论文

中文

分析试验室

1000-0720

2007

26

11

53-56

10.3969/j.issn.1000-0720.2007.11.014

湖南省自然科学基金（03JJY3030）项目资助；

本校为第一机构

建立了吖啶橙(AO)-罗丹明6G(R6G)共振能量转移荧光猝灭法测定尿中1-羟基芘的新方法.在λex/λem=470/556nm,十二烷基苯磺酸钠(SDBS)存在下,AO-R6G能够发生有效的能量转移,使R6G的荧光大大增强;1-羟基芘(1-OHP)的加入使R6G的荧光猝灭.方法的线性范围是21.3～982 μg/L;检出限为6.4 μg/L;平行7次测定相对标准偏差为0.98%～2.0%;回收率为96.0%～104.4%.该方法用于锅炉工尿样中1-羟基芘的测定,结果与常规的高效液相色谱法一致.

A novel method for the determination of 1-hydroxypyrene （1-OHP） in urine was established based on resonance energy transfer fluorescence quenching of acridine orange （AO）-rhodarnine6G （R6G）. It was found that the effective energy transfer could occur between AO and R6G in the presence of sodium dodecylbenzene sulfonate at λex/λcm = 470/556nm and the fluorescence intensity of R6G increased markedly. 1-OHP can extinguish the fluorescence intensity of R6G. The linear range of the calibration curve was 21.3 -982 μg/L and the detection limi...