Identification of a Constitutively Active Mutant Mouse IRAK2 by Retroviral Expression Screening

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成果类型：

期刊论文

作者：

Liu, Yanmei;Yin, Weilan;Xu, Lingqing;Zhang, Helin;Liu, Qian;...

通讯作者：

Yin, Weiguo

作者机构：

[Yin, Weiguo; Liu, Qian; Liu, Yanmei; Xu, Lingqing] Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Dept Clin Lab, Qingyuan 511518, Guangdong, Peoples R China.

[Yin, Weilan] Univ South China, Dept Physiol, Hengyang 421001, Hunan, Peoples R China.

[Yin, Weilan] Univ South China, Inst Neurosci, Hengyang 421001, Hunan, Peoples R China.

[Zhang, Helin] Shantou Univ, Coll Med, Yuebei Peoples Hosp, Dept Clin Lab, Shaoguan 512026, Guangdong, Peoples R China.

通讯机构：

[Yin, Weiguo] G

Guangzhou Med Univ, Qingyuan Peoples Hosp, Affiliated Hosp 6, Dept Clin Lab, Qingyuan 511518, Guangdong, Peoples R China.

语种：

英文

关键词：

Interleukin-1 receptor-related kinase-2;Retroviral vector;Gene recombination

期刊：

Molecular Biotechnology

ISSN：

1073-6085

年：

2018

卷：

期：

页码：

245-250

DOI：

10.1007/s12033-018-0064-9

基金类别：

Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [2015A030313691]; Public interest and ability construction funds in Guangdong Province [2014A020212036]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

To identify the importance of IRAK2 kinase activity in TLR-mediated signaling pathways, we constructed a retroviral vector harboring either a mouse IRAK2 gene (IRAK2-WT) or with its mutant with loss of function of its ATP-binding site (IRAK2-KD). Further, we comparatively analyzed for the gain of function and modulations in TLR-mediated signaling pathways in IRAK2 knockout (IRAK2-KO) macrophages upon introduction of the IRAK2-WT retroviral constructs. The pBS/IRAK2-KD with the ATP-binding site mutation in IRAK2 was obtained by using site-specific mutagenesis. The recombinants were identified w...

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Identification of a Constitutively Active Mutant Mouse IRAK2 by Retroviral Expression Screening

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