期刊论文

中文

中国公共卫生

1001-0580

2013

29

6

823-825

国家自然科学基金（81272994） 湖南省教育厅科研基金（12C0364）

本校为第一机构

目的探讨卷烟烟气抽提物(CSE)对支气管上皮细胞(BEAS-2B)遗传毒性及茶多酚干预作用。方法以高、中、低3个浓度对支气管上皮细胞进行染毒,同时设立茶多酚干预组和空白对照组;采用彗星实验及γ-H2AX蛋白表达检测DNA损伤,以微核实验检测染色体损伤,并检测HPRT基因突变率。结果高剂量染毒组BE-AS-2B细胞彗星细胞数(130.5±1.6)个、彗星尾长(134.33±3.56)μm、尾部面积(7 798.43±43.45)μm~2,均明显高于空白对照组(P

Objective To study the genetic toxicity in human bronchial epithelial cells（BEAS-2B） induced by cigarette smoke extraction（CSE） and protective effect of tea polyphenols（TPs） on the toxicity.Methods The BEAS-2B cells were exposed to high,moderate,and low concentration of CSE in culture medium and a tea polyphenols protection group and a control group were set up.Comet assay and H2AX phosphorylation were adopted to detect DNA damage and micronucleus assay was used to estimate chromosomal damage and hypoxanthine phosphoribosyl（HPRT） gene m...