期刊论文

中文

中国公共卫生

1001-0580

2009

25

6

733-735

10.3321/j.issn:1001-0580.2009.06.056

基金项目：国家自然科学基金（30572191） 南华大学科研基金（DS02224） 南华大学博士启动基金（5-2005-XQD-001）

本校为第一机构

目的 研究甲醛诱导肾上腺嗜铬细胞损伤特征及机制.方法 将肾上腺嗜铬细胞瘤细胞与不同浓度甲醛(20,40,80,160 μmol/L)共培养,24 h以后用四甲基偶氮噻唑蓝比色法检测细胞活力;Hoechst 33258荧光染色法检测细胞凋亡;分光光度法检测乳酸脱氢酶漏出量;硝酸还原酶法检测细胞上清液一氧化氮含量;生化法检测总一氧化氮合酶活性和诱导型一氧化氮合酶活性;磷酯酰丝氨酸结合蛋白-异硫氢酸荧光素/碘化丙啶双染法、流式细胞仪分析细胞死亡;免疫着色实验技术检测半胱氮酸蛋白酶-3前体表达.结果 20～160 μmol/L甲醛诱导细胞死亡,低剂量甲醛(80 μmol/L)主要诱导细胞坏死;细胞乳酸脱氢酶漏出量具有甲醛浓度依...

Objective To study the effect and mechanism of apoptosis of pheochromocytoma cells （PC12）induced by formaldehyde. Methods The viability of PC12 cells was detected by MTT. The apoptosis was measured by Hoechst 33258 fluorescence staining. The transudation content of LDH was determined by colormetry reaction. The nitrite content of the cul- ture medium was measured by nitrate reductase method. The apoptosis of NOS and iNOS were measured by biochemical method. The apoptosis of cells was determined by double-staining（Annexin-FITC/PI） and flow c...