期刊论文

Effect of MTBE on Glu and nNOS of brain tissues of fetal mice

中文

中国公共卫生

1001-0580

2008

24

5

577-579

10.3321/j.issn:1001-0580.2008.05.026

湖南省自然科学基金项目（06jj5052）；

本校为第一机构

目的 探讨大鼠妊娠期甲基叔丁基醚(MTBE)暴露对胎鼠脑组织神经元型一氧化氮合酶(nNOS)及神经递质谷氨酸(G1u)的影响.方法 40只健康成年昆明种小鼠于妊娠第0 d开始至妊娠结束期间,用食用油稀释的不同浓度MTBE 0,25,100,400,800 mg/(kg·bw)进行灌胃染毒,每天1次,直到胎鼠出生.用免疫组化的方法(SABC)观察胎鼠脑组织中nNOS和神经递质Glu免疫阳性神经元的表达.结果 当MTBE的剂量为400 mg/(kg·bw)时,胎鼠大脑皮质、海马和小脑nNOS和Glu阳性神经元细胞数较对照组减少(P＜0.05),染色强度变浅,并存在明显的剂量-效应关系.结论 妊娠期母鼠接触MTBE可致胎鼠脑组织nNOS及神经递质Glu的表达下降.

Objective To explore the effect of methyl tertiary- bitul ether （MTBT） on nNOS and neurotransmitter Glu of brain tissues of fetal mice during gestation. Methods 40 healthy and pregnant KM mice were distributed in five groups randomly. The MTBE exposed doses were 800,400,100 , 25 and 0 mg/（kg, bw） by garage from the 0 d to the end of gestation, once per day. The immunohisto - chemical method （SABC method） was used to check the expression of nNOS and neurotransmitter Glu in the brain tissues of the fetal mice. Results The expression of the ...