期刊论文

中文

中国公共卫生

1001-0580

2007

23

8

917-919

10.3321/j.issn:1001-0580.2007.08.010

本校为第一机构

目的 探讨已烯雌酚(DES)对人乳腺癌细胞(MCF-7细胞株)WISP-2基因(wnt-1 inducible signaling pathway protein-2)表达的影响及DES在乳癌发生中的作用机制.方法 用四甲基偶氮噻唑蓝(MTT)法检测DES对人乳癌MCF-7细胞生长活性的影响;以免疫细胞化学法及逆转录-聚合酶链反应法检测DES对人乳癌MCF-7细胞WISP-2 mRNA和WISP-2蛋白表达影响.结果 DES使MCF-7细胞增殖率增加,其吸光度A570值明显上升,与对照组比较,P＜0.05.以10nmol/L的DES分别作用细胞不同时间,A570值随着作用时间的延长而增加.DES可使MCF-7细胞WISP-2mRNA和WISP-2蛋白表达上升,WISP-2蛋白和mRNA的表达强度随药物浓度增加而升高,WISP-2 mR...

Objective To explore the effects of diethylstilbestrol on the expression of the WISP - 2 gene in human breast cancer cell line MCF - 7, and its mechanism. Methods To detect the proliferation by using MTT method. The expression of WISP- 2 mRNA and its protein were measured using RT - PCR and immunohistochemistry approcah. The experiment set up 17 - 13 estradiol as positive control. Results Diethylstilbestrol accelerated the MCF - 7 cell proliferation, the value of A570 obviously raised as the time raised, compared with control（ P 〈 0.05 ）;The...