期刊论文

中文

中国公共卫生

1001-0580

2005

21

2

149-151

10.3321/j.issn:1001-0580.2005.02.011

国家自然科学基金资助 （30 1 70 793）；

本校为第一机构

目的利用烷化剂的毒理机制,评价生长抑制和DNA损伤(Growth arrest and DNA damage,Gadd)基因的表达作为DNA损伤的分子标志物的可行性.方法通过烷化剂类药物氮芥诱导人肝瘤HepG2细胞,运用RT-PCR的方法检测Gadd基因的表达水平,同时佐以MTT试验、单细胞凝胶电泳试验来分析氮芥对细胞的毒性作用和DNA损伤程度.结果氮芥能诱导Gadd基因不同程度的表达,但随着浓度和时问的增加有由表达增强逐渐转为抑制的趋势;氮芥对人肝瘤HepG2细胞能产生明显的DNA损伤作用,有浓度依赖关系(rs=0.591,P＜0.01);氮芥对细胞有毒性作用,不同浓度的氮芥(0.5～40μg/L)使细胞存活率从97%下降到52%.结论Gadd基因的表达可以作为...

Objective To assess the feasibility of expression level of Growtharrest and DNA damage(Gadd )genes function as molecular biomarker of DNA lesion induced by nitrogen mustard,according to the toxicity mechanism of alkylating agents. Methods The human liver tumor HepG2 cells were first treated with nitrogen mustard,the mRNA level of Gadd45,Gadd 53,Gadd34 genes were determined by RT-PCR.Meanwhile the single cell gel electrophoresis (comet assay) was applied to determine the DNA damage induced by varying doses of nitrogen mustard and the 3-(4,5-meth...